8 Br cAMP on the monocytic cell line THP1 between ABCA1 and cell adhesion molecules.docVIP

 PAGE \* MERGEFORMAT 13 8 Br cAMP on the monocytic cell line THP1 between ABCA1 and cell adhesion molecules Of: Yong-Hong Yang Jiajun Hong WU Ping-sheng Guo Zhigang Li Jianhua [Abstract] Objective To observe the 8 Br cAMP stimulation in monocytic cell line THP1 ABCA1, intracellular adhesion molecule 1 (ICAM 1 and interleukin 1 (IL 1mRNA and protein expression changes, clarify the ABCA1 gene in atherosclerosis ( the possible formation mechanism of AS. Methods THP1 monocytes cultured recovery, adding 8 Br cAMP (0.5 mmol / L) stimulation 3,6,12,24 h, set at the same time without additional stimulation of the cells incubated for 24 h for the negative control group, by fluorescence quantitative RT PCR and Western blot and ELISA assay ABCA1, ICAM 1 and IL 1 mRNA and protein expression, with the ABCA1 antisense oligonucleotide (100 nmol / L transfected THP1 cells, to give 8 Br cAMP stimulation , the same changes in these indicators were observed. The results to 8 Br cAMP stimulation, THP1 cells, ABCA1, ICAM 1 mRNA and protein and IL 1 protein expression were increased, given antisense oligonucleotide transfected oxidized LDL protein (Ox LDL stimulation of 3,6 h mRNA expression of these indicators decreased (P lt;0.01), 12,24 h protein decreased (P lt;0.01). Conclusion THP1 monocytes, 8 Br cAMP activate ABCA1 not only increase cell Sinotrans cholesterol, also has the effect of increasing expression of inflammatory cytokines in the pathogenesis of AS, to play a dual role. [Keywords:] THP1 monocytes, ABCA1, atherosclerosis, inflammatory factors [Abstract] Objective To investigate the effects of 8 Br cAMP on ABCA1, ICAM 1, IL 1in human monocytes THP1 after the treatment with 8 Br cAMP.Methods 8 Br cAMP (0.5 mmol / L) were added into culture media and THP1 cells were harvested at 3,6,12 and 24 hours.The mRNA and protein levels of ABCA1, ICAM 1 and IL 1were investigated by real time fluorescent quantitative PCR, Western blot and ELISA methods.After phosphorothio