5 Dipsacus total alkaloid extraction and separation process of.docVIP

 PAGE \* MERGEFORMAT 12 5 Dipsacus total alkaloid extraction and separation process of [Abstract] Objective To study the 5 Dipsacus total alkaloids process. Methods The single-factor experiments and orthogonal experiment to study the Soxhlet extraction of total alkaloids Dipsacus process conditions, and ultraviolet spectrophotometry determination of total alkaloid content. The results obtained 5 Dipsacus best total alkaloid extraction conditions. Conclusion When the solid-liquid ratio of 1:20, extraction time 10 h, pH value of 3 ~ 3.5, 5 Dipsacus the highest rate of total alkaloid extract. [Keywords:] 5 Dipsacus single factor experiment the total alkaloid extraction and separation of orthogonal experiment Abstract: ObjectiveThis article studied the separation procedure on extraction of total alkaloids.MethodsThrough orthogonal experiment, the effect of the ration of solid-liquid, PH and time on extraction of total alkaloids were obtained, the contents of total alkaloids were determined by Arthrone coloimetry. ResultsProcedure Optimization of the extraction of the total alkaloids in Wu-He Dipsaus asper was obtained.Conclusion The optimization separation procedures were as follows; the ration between material and solvent is 1:20, the time is 10h, the PH is 3 ~ 3.5. Keywords:: Wu-He Dipsacus asper; Total alkaloids; Extraction; Separation procedure; Orthogonal experiment 5 Dipsacus Dipsacus asper are Dipsacaceae plants, perennial herb, medicinal parts of its dried roots [1]. 5 Dipsacus been processed with the roots of coarse, no end to end, quality is soft, dark green (commonly known as ebony color) daisy heart, gas micro-, taste slightly bitter, slightly sweet and astringent quality characteristics, especially the ‘Black Plum Mahogany’ the characteristics of known at home and abroad [2]. 5 Dipsacus main active ingredient saponins, volatile oils, alkaloids, vitamin E and sugar and so on, for the treatment of major clinical Yaoxisuanruan, bruises, fractures [3]