80μ m depth of excimer laser lamellar cutting preparation of photoreceptor cells.docVIP

 PAGE \* MERGEFORMAT 15 80μ m depth of excimer laser lamellar cutting preparation of photoreceptor cells Author: Li Gen-Lin Wang Gang, Wang Jin-Jin Lu Wenxiu Jing-Zhao [Abstract] Objective To investigate the PTK-cutting mode, the ideal preparation of excimer laser retinal photoreceptor cell lamellar optimal depth of cut required. Methods newborn calf retina as the research object, white and gelatin as carrier, the liquid film method retina Shop Shop films, excimer laser PTK-cutting mode, a total of three kinds of cutting depth of 48 photoreceptor cells prepared sheet layer by light microscopy and electron microscopy . Results of excimer laser PTK-cutting mode, 80μ m depth of cut and access to better lamellar retinal photoreceptor cells, light microscopy and scanning electron microscopy have shown that the complete segment of photoreceptor cells, outer granular layer, outer plexiform layer and some remnants of the within the granular layer cells, while the depth of 50μ m, and 100μ m, respectively shallow and deep. Conclusion Excimer laser PTK is a way 80μ m cutting lamellar retinal photoreceptor cells prepared by the appropriate depth of cut. [Keywords:] PTK mode excimer laser retinal photoreceptor cell layer of 80μ m depth of cut sheet . [Abstract] Objective To study the ideal ablation depth for harvesting sheets of photoreceptor cells from retina by using the excimer laser with PTK (phototherapeutic keratectomy, PTK) mode.Methods Retinas from neonatal calf, and gelatin as carrier were used.The retinas were located firstly and then laid on the surface of gelatin carrier using the devised liquid-phase method.The PTK mode of excimer laser was used to ablate retinal sheet.Total 48 photoreceptor sheets were ablated with three depths and were studied by light and scanning electron microscope. Results With 80 micron depth of ablation, photoreceptor cells were harvested as intact sheets from the retinas using the excimer laser.Light and scanning electron micr