921 traditional Chinese medicine extracts of anti-tumor mechanism of action.docVIP

 PAGE \* MERGEFORMAT 9 921 traditional Chinese medicine extracts of anti-tumor mechanism of action Of: the Bin Li, Li Hongyan, Guo Xiaojian, Chen Xiaoguang, Ye Zuguang, Ying-Lu Guo [Abstract] Objective To explore the crude extracts of traditional Chinese medicine compound 921 the mechanism of antitumor effect. Methods of biological macromolecules synthesis, cell cycle method, Western Blot method, testing extracts of Chinese herbal compound 921 on human prostate cancer cells in vitro PC -3M cell. Results 921 traditional Chinese medicine extracts on DNA synthesis in tumor cells inhibited the effect by 72 h after the cells were arrested in S phase can, and can induce the expression of S phase related proteins CyclinA down. Conclusions 921 side effect of anti-tumor mechanism of inhibiting tumor cell DNA synthesis, protein expression induced CyclinA down and thus cause cell cycle S phase arrest. [Keywords:] traditional Chinese medicine compound 921, anti-tumor mechanism of Keywords:: the extracts of prescription 921, anticancer activity, mechanism study 921 [1] by the black nightshade, Polyporus, Zedoary, Ligustrum lucidum, Vaccaria, psoralen composition. In the early experiments found that it has a certain effect in vivo and in vitro anticancer drugs [2] and less toxic. To further understand the anti-tumor mechanism of action, its experimental study. 1 Materials 1.1 Drugs Chinese medicine compound 921, the extraction of Curcuma essential oil dregs and Polyporus, Solanum nigrum mixed, water extraction and concentrated to thick paste, and the other taking psoralen, Vaccaria, Ligustrum lucidum extract to a thick paste with ethanol, the two merging, mixing, drying, grinding, made of particles, adding essential oil spray, sealed packaging, in a cool dry place. 1.2 cell line, reagent Prostate cancer cell line PC-3M, Institute of Urology, Peking University, saved passage, RPMI 1640 medium, trypsin, are GIBCO products, calf serum, Hangzhou E