A brother Wang genkwanin HPLC method for the determination.docVIP

 PAGE \* MERGEFORMAT 9 A brother Wang genkwanin HPLC method for the determination [Abstract] Objective To establish the brother Wang genkwanin determination method, in order to evaluate the quality of Costa Rica provide the basis for the king. Methods genkwanin HPLC method for the determination of its content, column: Alltech ODS (4.6 mm 250 mm, 5 m), mobile phase: methanol -0.2% phosphoric acid solution (volume ratio 55:45), flow rate 1.0 ml min-1, detection wavelength 346 nm, column temperature was 35 , the injection volume was 10 l. Results genkwanin in the range of 0.24 ~ 2.40 g and the peak area showed a good linear relationship, the regression equation Y = 84 069X +34.648, r = 0.999 8. The average recovery was 100.57%, RSD = 1.76% (n = 5). Conclusion The method is simple and accurate, good recovery, precision, high repeatability, rapid advantage of a brother king of herbs can be used as quality control. [Key Words] high performance liquid chromatography of the determination of Columbia Wang genkwanin Abstract: ObjectiveTo establish a method for the determination of the content of genkwanin in Wikstroemia indica (LCAMey (W.indica). MethodsThe content of genkwanin in W.indica was determined by HPLC with Alltech C_ (18) (250 mm 4.6 mm, 5 m) column. A mixture of methanol and 0.2% phosphoric acid solution (55:45) was used as mobile phase, the flow rate was 1.0 ml min-1 and UV detection wavelength was at 346 nm. Temperature was 35 and sample size was10 l.ResultsThe linear relationship was good within the range of 0.24 ~ 2.40 g (r = 0.999 8). The average recovery was 100. 57%, RSD was 1.76%. ConclusionThe method is simple and accurate with good separation. The results are sensitive and reproducible and may be used in the quality control of genkwanin in Wikstroemia indica (LCAMey. Keywords:: HPLC; Wikstroemia indica; Genkwanin, Determination A brother king of traditional Chinese medicine for the plants of the Columbia Daphne King Wikstroemia