A white-backed leaf extract on proliferation of human tumor cell research.docVIP

 PAGE \* MERGEFORMAT 7 A white-backed leaf extract on proliferation of human tumor cell research [Abstract] Objective To observe the white-back A leaf extract on tumor cell proliferation. Methods colorimetric MTT test (MTT method, the half inhibitory concentration (IC50 for the evaluation of the strength of anti-tumor activity index, respectively, 4 common human cancer cells to antitumor activity screening. Results A white-backed leaf extract at a concentration of 5 g / ml of human gastric cancer cells when SGC-7901, A549 human lung cancer cells significantly inhibited (P lt;0.05 or P lt;0.01, IC50 were 27.14 g / ml, 33.08 g / ml, at a concentration of 20 g / ml LoVo human colon cancer cells when inhibited (P lt;0.05, IC50 = 36.82 g / ml, at a concentration of 40 g / ml when Bel-7404 human hepatoma cells inhibited (P lt;0.05, the IC50 was 37.70g/ml. Conclusion A leaf extract of white on the back above 4 inhibited tumor cell proliferation. [Keywords:] white dorsal leaf extract A, anti-tumor, MTT colorimetric test White apelta Mallotus apelta Muell-Arg. Dicotyledon herbal Euphorbiaceae for the white catalpa leaves of plants, also known as white-backed mother, white-backed Tong, Lingnan Folk commonly used herbs. Widely distributed in China, resources are abundant, can be found in Guangdong, Guangxi, Hunan, Fujian, Zhejiang, Anhui, Henan and other places. Mallotus leaves can be taken throughout the year, fresh or dried. color map of China recorded its strong bitter medicine, nature, with heat, detoxification, dampness, the effect of bleeding [1]. In this study, leaf extract A white background was the screening of anti-tumor effect. now the results reported below. 1 Materials and equipment 1.1 Drugs A white-backed leaf extract extracted by the Department of Chemistry, Guangxi University, isolated, light yellow monomer. 1.2 Tumor cell lines Cell lines used in this study were purchased from Bio Co., Ltd. Nanjing KGI. 1.3 Reagents RPMI-164