Acanthopanax polysaccharides on hydrogen peroxide-induced oxidative stress injury in hippocampal neurons and cell apoptosis-related gene family of -2.docVIP

 PAGE \* MERGEFORMAT 10 Acanthopanax polysaccharides on hydrogen peroxide-induced oxidative stress injury in hippocampal neurons and cell apoptosis-related gene family of -2 Author: Diao Bo Tang Ying De-Zhong Li Denghui Ling-Wen Ye Wang Xiaokun [Abstract] Objective To observe the Acanthopanax polysaccharides (ASPS) on H2O2-induced oxidative stress injury in hippocampal neurons and cell apoptosis-related genes -2 (Bcl-2) family influence, and to explore its possible mechanism. Methods hippocampal neurons in primary culture technique, using H2O2-induced oxidative injury model of the establishment of cells. Morphological changes of the cells were observed; MTT determination of cell activity; TUNEL method to detect H2O2-induced apoptosis in rat hippocampal neurons; Immunohistochemistry was used to detect cells in Bcl-2, Bax protein expression; chemical colorimetric assay of cell homogenate of nitric oxide synthase (NOS), superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. The results of morphological observation showed that: Compared with model group, ASPS group of cell damage is reduced significantly; ASPS group of cell viability compared with model group, cells with high activity; TUNEL assay showed positive cells ASPS pretreatment group was significantly higher than in model group cells; ASPS group of Bcl -2 expression was higher and lower Bax expression; NOS activity and the amount of MDA generated ASPS group was significantly lower than that in model group, but SOD activity was significantly higher than the ASPS model group. Conclusion ASPS can enhance hippocampal neurons against oxidative stress injury in capacity and increase Bcl-2 gene expression, reduced Bax expression. [Keywords:] Acanthopanax polysaccharides hippocampal nerve cells from oxidative injury in an emergency -2 family of apoptosis-related genes Free radicals are a class of non-paired electrons active chemical groups. Oxygen free radicals and nitrogen in which oxygen free