Acellular natural scaffold spinal cord and morphological observation.docVIP

 PAGE \* MERGEFORMAT 21 Acellular natural scaffold spinal cord and morphological observation Author: Guo Zhang Ren Xian-jun JIANG Tao Wu Bo Abstract [Objective] using chemical methods to remove cells and off cells produce myelin components of extracellular matrix scaffold for bridging defects in spinal cord injury provides an ideal natural nerve scaffold. [Method] SD rat thoracic spinal cord is about 2 cm, the use of freeze-thaw chemical extraction (3% sodium deoxycholate, and 1 KU / ml DNaseI, RNaseA) for tissue engineering approach to rat spinal cord tissue, and after treatment spinal cord bracket respectively histological examination, the liberation of cells and extracellular matrix scaffold patterns. [Results] after acellular processing, light microscope, HE staining of spinal cord cross-section of reticular structure, and no cellular components retained, sagittal plane showed interwoven tubular pathway; no axon, myelin sheath and nucleus. Myelin staining showed complete removal of myelin, no myelin components. [Conclusion] In this study, use of freeze-thaw method of chemical extraction of tissue engineering can be the ideal preparation of the natural spinal cord stents, the stents and spinal cord three-dimensional tissue structure has a high degree of similarity, is expected to spinal cord injury as a bridging material and nerve tissue engineering stent, the experimental results showed that 3% sodium deoxycholate, and 1 KU / ml DNaseI, RNaseA for acellular deal with two is more appropriate, and can be more thoroughly remove the cells and myelin components, and relatively intact extracellular matrix The three-dimensional structure. Keywords: acellular rat spinal cord tissue engineering scaffolds Preparation of acellular scaffold of natural spinal cord and observation of morphology Abstract: [Objective] Using a procedure of chemical agent to remove the cells and myethin in spinal cord of rat and to prepare the scaffold of ex