Acorus gramineus Determination of total amino acids.docVIP

 PAGE \* MERGEFORMAT 9 Acorus gramineus Determination of total amino acids [Abstract] Objective To establish a total of Inner Mongolia Shichangpu method for the determination of amino acids. Methods spectrophotometry. The results of the standard curve equation A = 0.001 1C-0.019 9 (r = 0.999 3), the average recovery was 97.10%, RSD of 0.95% (n = 6). The method measured the total amino acid content of Acorus gramineus 0.62%, with the amino acid analyzer measured the total amino acid content of Acorus gramineus line. Conclusion The method is sensitive, simple, rapid, accurate and reliable. [Keywords:] Shichangpu; total amino acids; spectrophotometry Abstract: ObjectiveTo establish a method for determination of total amino acid in A.tatarinowii Schott. MethodsUltra Violet-Visible absorption spectrophotometry was adopted.ResultsThe standard curve was A = 0.0011C-0.0199, r = 0.999 3, and the average recovery was 97.10% , RSD = 0.95% (n = 6). ConclusionThe guantitative method is simple, rapid and reliable. Keywords:: A.tartarinowii Schott.; Total amino acid; Spectrophotometry Department of Araceae Acorus gramineus (Araceae) Acorus (Acorus) Shichangpu A.tatarinowii Schott.’s Dry roots. A Huashi appetizer, resuscitation Huo Tan, Xingshen puzzle and other effects [1]. Can be used to Zhongwan swelling of the liver is not hunger, Shenhun epilepsy, forgetfulness deafness [2] and other diseases. Are reported in the literature [3] Shichangpu contains amino acids, but the total amino acid content determination of which have not been reported. In this paper, Determination of Shichangpu total amino acid content of herbs in the method, this method is simple, fast, recovery is 95% ~ 105%. 1 Materials and equipment 1.1 Material Shichangpu Chinese medicinal herbs (herbs from the Tong Ren Tang wholesale offers, Origin: Hunan, production batch number: 401,005,732, after Beijing University of Chinese Medicine Traditional Chinese Medicine College of Medicine Department of Healt