Acute spinal cord injury the expression of heat shock protein 47 changes.docVIP

 PAGE \* MERGEFORMAT 9 Acute spinal cord injury the expression of heat shock protein 47 changes Study: How bao Yang Chen Yang Yougeng Ren Xiansheng Abstract Objective To study the aged rats with acute spinal cord injury (SCI) in the heat shock protein 47 (HSP47) expression. Methods 54 Wistar rats was clamped type of SCI model, the 3 d after SCI and weekly evaluation of BBB score, and 1,3,5 w analyzed by immunohistochemistry HSP47 expression in the spinal cord. Results In normal Wistar rat spinal cord tissue with low expression of HSP47, the expression was significantly increased after SCI, and in the first 5 weeks there is still an upward trend (P lt;0.01). Gray matter after spinal cord injury and white matter boundaries unclear, apoptosis significantly, HSP47 mainly in the meningeal blood vessels and close to the soft expression at the white matter. Conclusion old Wistar rats after SCI increased expression of HSP47, HSP47 may participate in the process of the pathological changes of SCI. Keywords: acute spinal cord injury; heat shock protein 47; clamp model of acute spinal cord injury Elderly patients between the spine and disc degeneration, spinal cord injury (SCI) higher cancer risk. Glial scar repair spinal cord injury is one of the factors impeding 1, and heat shock protein 47 (HSP47) in the process of scar formation play an important role in 2. But after HSP47 expression in the SCI literature has not been reported. This research study by immunohistochemistry HSP47 changes after SCI, and explore a new target for treatment of spinal cord injury. 1 Materials and Methods 1.1 Animals Wistar rats (clean grade) 54, 12 months old rats, weighing 200 ~ 250 g, Experimental Animal Center of Jilin University. 1.2 Reagents and instruments HSP47 (1:200, Santa Cruz Company), glial cell acidic protein (GFAP, 1:50), neurofilament (NF, 1:100, Wuhan Boster Biological Engineering Co., Ltd.), UltraSensitiveTMS P ultra-sensitive kit (mouse / r