Adipose derived stem cells to repair full-thickness cartilage defects in rabbits of knee.docVIP

 PAGE \* MERGEFORMAT 19 Adipose derived stem cells to repair full-thickness cartilage defects in rabbits of knee [Abstract] observe the evaluation of the patellofemoral joint surface of rabbit knee joint center of artificial full-thickness articular cartilage defects, the pure fat source of calcium alginate gel stem cells transplanted into articular cartilage defects of the repair of full thickness cartilage defect effect, to explore joint cartilage defect repair simple and effective way. [Method] 27 New Zealand white rabbits were randomly divided into A, B, C three groups, A group of articular cartilage defects disposal into calcium alginate gel, B group without any treatment of cartilage defects, C Group disposed of into the defect without the fat derived stem cells induced by calcium alginate gel compound, 4,8,12 weeks after operation, respectively, animals were killed by gross, histological observation and transmission electron microscopy techniques to observe the effect of repair , using the modified Wakitani score to rate the standard of repair tissue obtained score using statistical software SPSS 11.5 for statistical analysis. [Results] A, B group of the cartilage defect was filled with fibrous tissue tilting, histological sections can be seen covering the original fiber , C group for the class of cartilage defects of hyaline cartilage tissue filling, cartilage cells are seen in tissue sections and around the lacunae, electron microscope, a large number of cells around collagen fibers, C group in each period and A, B group differences in scores between are statistically significant (P lt;0.01), group C showed two sets of fixed effects is superior than the other. [Conclusion] adipose derived stem cells combined with pure calcium alginate gel can be an effective full-thickness defects of articular cartilage repair. [Keywords:] adipose derived stem cells in cartilage calcium alginate Abstract: [Objective] To evaluate the effect of not induced adipo