Advanced glycation end products on rat vascular smooth muscle cells in OPG mRNA expression in.docVIP

 PAGE \* MERGEFORMAT 17 Advanced glycation end products on rat vascular smooth muscle cells in OPG mRNA expression in Authors: Guo Xiufang, REN Xiao-mei, Wei Qin, LIU Nai-Feng 【Abstract】 Objective: To study the advanced glycation end products (AGEs) on rat vascular smooth muscle cells (VSMCs) osteoprotegerin (OPG) mRNA expression. Methods: The cultured VSMCs, containing 10mmol * L-1β-glycerol phosphate (β-GP) in the culture medium with different concentrations (0,50,100,200,400 mg * L-1) of the AGE-bovine serum albumin (BSA) were incubated with VSMCs at different time (0,12,24,36,48,72 h). Using RT-PCR, the mRNA expression levels of OPG. Results: AGE-BSA was time-and dose-dependent increase VSMCsOPGmRNA expression; 200mg * L-1 of the AGE-BSA and the VSMCs were incubated 24h, OPGmRNA expression level reached a peak. Conclusions: AGE-BSA can promote the expression of rat VSMCsOPGmRNA. Keywords: advanced glycation end products; vascular smooth muscle cell; calcification; OPG; rat Cardiovascular disease is diabetes mellitus (diabetes mellitus, DM) in patients with major causes of disability in death, vascular calcification is its pathophysiological basis of [1], to predict cardiovascular events in DM patients with the most appropriate indicators [2]. Non-enzymatic glycation of protein formation of advanced glycation end products (advanced glycosylation end products, AGEs) in mediating the high glucose-induced cardiovascular disease in DM play an important role in the process [3]. Recent studies have shown that OPG (osteoprotegerin, OPG) has become the field of bone metabolism and artery calcification of major discoveries and new topics. Detection of OPG may serve as indicators for the early diagnosis of vascular calcification, one of [4]. The experimental observation of AGEs in cultured rat vascular smooth muscle cells (vascular smooth muscle cells, VSMCs) calcification of the impact in order to explore the mechanisms of vascular calcificat