After re-hydration of freeze-dried platelet aggregation.docVIP

 PAGE \* MERGEFORMAT 3 After re-hydration of freeze-dried platelet aggregation Authors: Jing-Han Liu, Lu Fa-Qiang *, Zhuang Yuan, car, Series A, Chen Lin Feng [Abstract] This study was designed to observe the re-hydration of freeze-dried platelet aggregation response after the changes. In 4 different concentrations of thrombin, Ristocetin, ADP, collagen as inducing agent, APACT2 platelet aggregation meter, test re-hydration of freeze-dried and fresh platelet maximum aggregation rate in order to ADP as the inducer, detection cellular re-hydration of freeze-dried trehalose inside and outside the maximum platelet aggregation rate. The results showed that: When thrombin, Ristocetin, ADP, collagen concentrations were 1 U / ml, 1.6 mg / ml, 20 μ mol / L, 2 μ g / ml, the new maximum platelet aggregation rate of 100% or so, Freeze-dried rehydrated platelet aggregation rates were (70.17 ± 7.36)%, (15.3 ± 2.81)%, (68.67 ± 6.86)%, (64.67 ± 11.6)%. Inside and outside extracellular trehalose group, extracellular trehalose group and blank control group, re-hydration of freeze-dried platelet aggregation rates were (66.0 ± 4.69)%, (25.3 ± 2.42)%, (11.5 ± 1.87)% (P amp;lt;0.01 ). Conclusion: The thrombin, Ristocetin, ADP, collagen concentrations were 1 U / ml, 1.6 mg / ml, 20 μ mol / L, 2 μ g / ml of platelet aggregation test can be used as a suitable concentration of intracellular trehalose inside and outside so that Freeze-dried rehydrated platelet aggregation was significantly increased. [Keywords:] re-hydration; freeze-dried platelets; trehalose; platelet Aggregation after Rehydration of Lyophilized Platelets Abstract This study was aimed to investigate the aggregation of rehydrated-lyophilized platelets. The aggregation rate of fresh and rehydrated-lyophilized platelets were measured by using thrombin, ristocetin, ADP and collagen as inductors and APACT2 aggregameter; the effects of intra-and extra - cellular trehalose on maximum aggregation rate of rehydrate