Animals and healthy human gut symbiotic E. coli causes resistance and Analysis.docVIP

 PAGE \* MERGEFORMAT 20 Animals and healthy human gut symbiotic E. coli causes resistance and Analysis Author: LI Jing-yun Yue Cui Sheng-Hui HU Chang-Qin Ma [Abstract] Objective To understand the current animal and human intestinal symbiotic resistance of Escherichia coli, analysis of its causes. Method from Gansu, Hubei, Beijing, Shandong, Sichuan and other places relatively closed fish farms and fish farms in the vicinity of the health of people collecting chickens, pigs, fish, human stool samples, separation of Escherichia coli. Be identified with the identification of API20E suspected of Escherichia coli bacteria, using KB disk diffusion method for detection of biochemical identification of drug-resistant strains of Escherichia coli, using software WHONET5.3 susceptibility test data analysis. Using pulsed-field gel electrophoresis detection of similar resistant patterns of homology among strains of Escherichia coli. Results ① 571 E. coli were collected, of which farmed fish from sea water and freshwater fish isolated 31, and the other from the sea fish and freshwater fish isolated Aeromonas hydrophila strain 57; ② source of chicken Detection of Escherichia coli on all the highest rates of antimicrobial agents; ③ In addition to Β  lactam antibiotics and amikacin, the chicken, pig and human sources of strains showed resistance rates of high, medium and low phenomenon, some of the old quinolone antibacterial drugs and is particularly evident; different regions of isolated resistance also have been quite different; ④ The survey found that for the first time in the domestic chicken farm ESBL producing E. coli, and very prevalent; ⑤ Aeromonas hydrophila and Escherichia coli resistance to a larger difference in 47 resistant patterns similar to strains found in levels of three groups of genes homologous strains (similarity greater than 95 %). Conclusions ① from different regions and different types of resistance of animal isolates from different, and rel