Application of PCR method for rapid identification of pathogenic fungi in clinical specimens of body fluids.docVIP

 PAGE \* MERGEFORMAT 9 Application of PCR method for rapid identification of pathogenic fungi in clinical specimens of body fluids [Abstract] Objective PCR method for rapid identification of clinical pathogenic fungi in the body fluid samples. Methods fungal universal primers ITS4 and ITS86 body fluid specimens for clinical pathogenic fungi in the Rapid Identification of PCR amplification products of the corresponding strain with the control group DNA was extracted using the traditional method of scanning after the analysis of amplified fragment contrast. The results of clinical body fluid specimens were PCR amplified directly scan results with the results of DNA amplification similar, the difference was not statistically significant. Conclusion universal primers ITS CLINICAL body fluid samples, PCR, identification of pathogenic fungi, accurate, specific, rapid and sensitive detection of pathogens. [Keywords:] deep fungal infections; clinical body fluid specimens; PCR In recent years, the incidence of deep fungal disease by more than the rate of increase in the past 20 years. The mortality rate of infection as high as 30% to 60%, 36% of the organizations involved patients with true bacteremia, the mortality rate of 47% to 88 %, by certain fungi such as Aspergillus and Fusarium disseminated fungal infections are caused by the mortality rate approaches 100% [1]. Early diagnosis of deep fungal infections have a significant impact on clinical outcome, in more and more resistant strain appears, the importance of species identification has been ignored. due to the current fungus identification also depends on the morphology of many fungi after a few days or even weeks of training received only genus identification, still need to be identified to the species through complex biochemical experiments and Immunology, and most bacteria, in particular filamentous fungi only by experienced technical staff can identify. Therefore, we Jiezhu Yu PCR identification of