Arsenic trioxide induced apoptosis in gastric cancer cells Bcl-2 phenotype of the significance of changes in.docVIP

 PAGE \* MERGEFORMAT 12 Arsenic trioxide induced apoptosis in gastric cancer cells Bcl-2 phenotype of the significance of changes in Of: ZHENG Hu, Zhou Meng Tao, single Yunfeng, Zhang Qiyu [Abstract] Objective: To investigate the arsenic trioxide (As2O3) is able to induce apoptosis of SGC7901 gastric cancer cells, and Bcl-2 whether the phenotypic changes played a role. Methods: The effect of different concentrations of arsenic trioxide in SGC7901 cells by CCK-8 the cytotoxic test, using DAPI staining of nuclear morphology, observed by immunofluorescence microscopy phenotype changes in the expression of Bcl-2 by Western Blot Western blot detection of Bcl-2 expression. Results: Arsenic trioxide can induce withered SGC7901 cells death. trioxide induced apoptosis in SGC7901 cells, Bcl-2 associated with the change of phenotype and the expression of Bcl-2 did not change. CONCLUSION: Arsenic trioxide by Bcl-2 may change in phenotype induced apoptosis of SGC7901 gastric cancer cells, rather than through The expression of Bcl-2 down. [Keywords:] arsenic trioxide apoptosis in gastric cancer cells Bcl-2 Abstract: Objective: To explone the effects of arsenic trioxide (As2O3) on induction of apoptosis in SGC7901 human gastric cancer cells and the phenotype change of Bcl-2. Methods: SGC7901 human gastric cancer cells were treated with different concentrations of arsenic trioxide. Cytotoxicity was measured by using the Cell Counting Assay Kit-8. Analysis of nuclear morphology was observed with DAPI staining and the phenotype change of Bcl-2 was observed under the immunofluorescence microscopy. The expression of Bcl-2 was examined with Western Blot analysis. Results : Arsenic trioxide could induce apoptosis in SGC7901 human gastric cancer cells, and the apoptosis was accompanied with phenotype change of Bcl-2, but arsenic trioxide did not affect expression of Bcl-2. Conclusion: Arsenic in SGC7901 cells induced by arsenic trioxide may be expressed through the indu