Articular cartilage chondrocyte apoptosis following impact injury in experimental study.docVIP

 PAGE \* MERGEFORMAT 12 Articular cartilage chondrocyte apoptosis following impact injury in experimental study Authors: Shao-kun, Fu-Xing Pei, Di Dong-hua, Zhao Jianzhong 【Abstract】 Objective] with the TUNEL labeling method and transmission electron microscopy to observe the cartilage after impact injury in the evolution of chondrocyte apoptosis. [Method] 56 healthy adult New Zealand white rabbits were randomly divided into high and low energy groups, each of 28 randomly selected side of the hind limbs of the experimental group, on the other side of the control group; different weights of the hammer hit the inside of the femur condylar cartilage damage caused by the different. After 4 d, 1,2,4,8,16,32 weeks to collect specimens, each time group 4 rabbits, carried out TUNEL labeling method and transmission electron microscopy to observe the apoptosis of chondrocytes. [Results] In the control group, specimens from the middle layer and calcified cartilage layer, apoptotic cells. Low-energy group, after injury 4 d, 1,2-week specimens, cartilage intermediate layer of the transitional layer and apoptotic cells, the apoptotic rate between the control group had no statistical significance; 4 weeks after the specimen significantly reduced in apoptotic cells, apoptosis rate were not statistically significant between groups. High-energy group, 4 d after injury cartilage surface and transitional layer of light appears in apoptotic cells, and the injury the longer the greater the percentage of apoptotic cells, apoptosis rate were statistically significant between groups . [Conclusion] cartilage high, low-energy impact injuries suffered from an early chondrocyte apoptosis, apoptotic rate was higher; but low-energy injury 4 weeks after chondrocyte apoptosis returned to control levels; high-energy injury cartilage apoptosis rate increased gradually over time. Keywords: cartilage; cartilage cells; apoptosis; traumatic osteoarthritis Recent studie