Asiatic acid on cell proliferation of liver cancer.docVIP

 PAGE \* MERGEFORMAT 15 Asiatic acid on cell proliferation of liver cancer Of: Zhou Jun, Gao Jing, Fang Chun money, Na Lin, Yan Lifang [Abstract] Objective: To study the asiatic acid (asiatic acid, AA on human hepatoma cell line (HepG2 inhibition, and to explore the relationship between mitochondria. Methods: MTT assay AA on HepG2 cell proliferation, inverted microscope morphological changes in the number of fluorescent probe MitoTracker mitochondrial fluorescent probe JC 1 mitochondrial membrane potential, fluorescent intracellular probe DCFH DA detection of ROS (reactive oxygen species, ROS content, ATP intracellular kit ATP level; RT PCR and Western Blot Analysis of voltage-dependent anion channel (voltage dependent anion channel, VDAC expression. Results: AA to dose-dependent inhibition of tumor cell proliferation, 30 mol / L AA role in cells 24 h disappeared after the cell processes, cell body shrinkage, rounding. AA can make HepG2 cells decreased mitochondrial membrane potential, intracellular ROS content increased, decreased intracellular ATP level, 50 mol / L AA can increase the amount of VDAC protein expression. Conclusion: AA inhibition of tumor cell proliferation and mitochondrial related to mitochondrial VDAC in apoptosis of human hepatoma cell process. [Keywords:] asiatic acid; mitochondria; voltage-dependent anion channel; hepatoma cell line [Abstract] Objective: To investigate the proliferation inhibition of asiatic acid (AA) on the human hepatoma cell line (HepG2) cells, and to study its potential correlation with mitochondria.Methods: The effect of AA on proliferation of HepG2 was tested by MTT , morphology of tumor cells was observed under inverted microscope; the mitochondrial mass was described by the fluorescence intensity of MitoTracker probe, mitochondrial membrane potential was detected with fluorescent probe JC 1, intracellular reactive oxygen species (ROS) content was detected with DCFH DA, intracellular ATP level was measu