Atmospheric pollutants on the lung tissue of rats with CC16 and cytokine mRNA expression in.docVIP

 PAGE \* MERGEFORMAT 6 Atmospheric pollutants on the lung tissue of rats with CC16 and cytokine mRNA expression in Authors: Xiao Chun Ling, Guo, QI Rong, Xi Shu-Hua, Wang Ren Qun [Abstract] Objective: To determine the atmospheric pollutants on the lung tissue CC16, TNF  α and IL  6 of the mRNA expression of the impact of atmospheric pollutants on lung injury in rats. Methods: The low-flow made in Japan PM10 air samplers collected particulate matter PM10, sampling membrane eluted with normal saline ultrasonic vibration, suspension fixed volume of 15mg/ml. 48 body weight 200 ~ 240g Wistar rats were randomly divided into three experimental groups and one control group. The experimental group rats were injected into the trachea of saline 1ml 15mg/ml PM10 suspension, 1ml normal saline injected control rats. The next day, a static experimental rats inhaled concentrations were 15,12,400 mg/m3 of SO2, NO2, CO air mixture, a daily inhaled 2h, the control group inhaled normal air. Inhalation of gaseous pollutants in the 1d, 7d, and 30d day after the experimental group were sacrificed and the control rats, lung tissue, using RT-PCR, determination of TNF  α , IL  6 and the mRNA expression levels of CC16. Results: The inhalation of air pollutants Unit 1d, and 7d after inhalation of its expression in lung tissue CC16mRNA was significantly lower than the control group; cytokines TNF  α and IL  6 mRNA expression increased, higher and was exposed to an early stage Exposure Cap 1d and 7d increased significantly, exposed to the first 30d, also showed a downward trend. Conclusion: The role of atmospheric pollutants on the respiratory system in rats, the expression of rat lung tissue CC16mRNA decreased, TNF  α and IL  6 mRNA expression increased, and the role of the early changes of pollutants significantly. [Keywords:] air pollutants; rat; Clara cell secretory protein; TNF  α ; IL  6, mRNA AbstractObjective: To study mRNA expression of CC16, TNF  α