Automatic sampler plus an analysis of the phenomenon of positive pollution.docVIP

 PAGE \* MERGEFORMAT 6 Automatic sampler plus an analysis of the phenomenon of positive pollution [Keywords:] Automatic Automatic sample handling instrument has been widely used in the major blood banks, the author found in the application appears strong positive syphilis test samples contaminated rear hole phenomenon, and manual review of the back hole and the pigtail tube specimens were negative, these are as follows. 1 Materials and methods 1.1 Specimen Source Serum samples from blood donors are unpaid. 1.2 Reagents Syphilis reagents for Shanghai Branch China Bio-engineering Co., Ltd. and Beijing China down than the love of Bio-Technology Co., Ltd. production. 1.3 Instrument TECAN RSP plus injector. 1.4 Methods In strict accordance with the instructions required reagent plus sample editing program, RSP sampler automatically increase the formation of the experimental samples before and after the increase in order not to artificially change detection of 88 specimens of each plate. Check the beginning of the order of adding sample HBsAg, anti-HCV, ALT, anti-HIV, TP (Shanghai Branch China ELISA), or review the order of adding sample of anti-HIV, anti-HCV, HBsAg, TP (Beijing Ji than love ELISA) , ALT, eight Canadian-like needles the first row of the first inhaled in sufficient quantities of serum samples, followed by adding five experiments ELISA plates, add the kind of needles washed twice inside and outside, together with the specimens of the second row, and so on. Canada finished samples, the ELISA plate into FAME automatic analysis system for testing. Appears positive, the pairs of positive samples were then manually reviewed pigtail and in vitro. Pollution discharged from 23 specimens collected, a total of 10 groups of these samples and normal samples by group, composed of a mixing board 88 copies of each specimen within the ELISA plate before and after in the order of the same, with the TECAN RSP plus injector for each an increase of only a syphi