Bacteroides fragilis fluorescence quantitative PCR assay.docVIP

 PAGE \* MERGEFORMAT 7 Bacteroides fragilis fluorescence quantitative PCR assay [Keywords:] Bacteroides fragilis; fluorescence quantitative PCR; SYBR, Green, I Abstract: Objective To investigate the fluorescence quantitative PCR detection of Bacteroides fragilis. Methods using specific primers and fluorescent dye real-time PCR amplification and detection products. Results Bacteroides fragilis reference strain (ATCC 25285) and the four isolates were specific amplification curve, sensitivity up to 102 bacteria; while Escherichia coli, Lactobacillus bulgaricus, Streptococcus thermophilus non-specific amplification curve. Conclusion fluorescent dye real-time PCR technology can be specific and quantitative detection of Bacteroides fragilis Keywords: Bacteroides fragilis; fluorescence quantitative PCR; SYBR Green I Detection bacteria fragile by SYBR Green real-time PCR Abstract: Objective To identify the bacteria fragiles (B.fragiles) by 16S target rRNA realtime PCR.Methods A pair of specific primers was designed.The PCR products were detected by fluorescent quantificative realtime PCR.Results The B.fragilis ATCC 25285 and 4 separative strains of B.fragilis appeared in the special amplification product curve, while E.coli, L.bulgaricus and S.thermophilus did not.The flourescent qantificative PCR can detect 102 bacteria.Conclusion 16S rRNAtargeted primer and fluorescent quantifive realtime PCR can be applicable for the identification and quantification of B.fragilis. Keywords:: bacteria fragile; fluorescent quatititive PCR; SYBR Green I Bacteroides fragilis is a G-sex non-spore anaerobic bacteria of clinical isolates of the most common, conventional anaerobic culture required separation of 2 ~ 7d, can not be timely identified to guide the clinical pathogens. Bacterial development of molecular biology techniques to provide early detection of bacterial infection of specific and sensitive detection methods 〔 1〕 . In this paper, fluorescence quantitative PCR dete