Bone marrow stromal cells in the rat model of striatal hemorrhage survival and migration of brain.docVIP

 PAGE \* MERGEFORMAT 17 Bone marrow stromal cells in the rat model of striatal hemorrhage survival and migration of brain [Abstract] Objective To observe bone marrow stromal cells (MSCs) in the rat model of intracerebral hemorrhage survival in the striatum and migration features to explore the possibility of transplanting brain hemorrhage and suitable conditions. Methods were isolated by density gradient centrifugation of adult SD rat MSCs, flow cytometry to detect cell surface antigens CD11b, CD45 and CD90 to determine its purity, a large number of amplified cells with retroviral vector transfection of enhanced green fluorescent protein (EGFP) marker gene. collagenase Prepared by striatal injection of heparin ICH models, behavioral testing is successful, the cell suspension 5L stereotactic implantation hemorrhage rat model of cerebral hemorrhage around. batches at different times to kill rats irrigation, and the brain as a frozen sections, immunohistochemistry detected EGFP expression in transplanted cells. The results detected by flow cytometry CD90 + / CD45-/CD11b- cells reached 75%. cells in vitro to stably express EGFP.MSCs hemorrhage in the brain can survive for at least around 4 weeks, these cells varied shapes, mainly in the peripheral hemorrhage, migration to the surrounding brain parenchyma, most of the cells to settle in around the vessels. Conclusion of high purity isolated and cultured MSCs, proliferation, and can be efficiently expressed EGFP, transplanted to the brain hemorrhage around the migration to the surrounding viable. [Keywords:] intracerebral hemorrhage survival of striatal rat bone marrow stromal cell transplantation Abstract: Objective To explore the surviving and migration of Bone Marrow Stromal Cells (MSCs) in brain of the striatal hemorrhage model of rat, and to investigate the possibility and appropriate condition of transplanting MSCs into brain of the striatal hemorrhage model. Methods MSCs was collected from adult Sprag