China Association of Pharmaceutical Biological Chemistry bone bank branch of the trial acellular nerve repair nerve defect length of the experimental study.docVIP

 PAGE \* MERGEFORMAT 2 China Association of Pharmaceutical Biological Chemistry bone bank branch of the trial acellular nerve repair nerve defect length of the experimental study Author: Ming-Xue Sun, Lu Shi-bi, Tang Jin-Shu, WANG Xin, Zhao Bin, SUI Xiang, Xu Wenjing, Zhang Li [Keywords:] Allograft Abstract: [Objective] To study whether the impact of chemicals to the length of nerve cells, nerve and chemical quality of acellular nerve repair nerve defects in dogs the proper length range. [Methods] dog sciatic nerve were cut length, cutting diameter of approximate lengths 12 cm, to remove the fat tissue of epineurium and blood vessels, in 50% Triton X100 and 50% sodium deoxycholate repeated chemical digestion to be acellular nerve; part of the nerve for histological evaluation, will be 12 cm nerve cut into 6 sequence, embedded in paraffin and preparation of slices, the thickness of 5 μ m. HE staining and Fastblue staining to the cell level, nerve extracellular matrix structural integrity of the myelin sheath staining intensity observed differences in each piece of nerve. In vivo experiments, with chemical acellular allogenic nerve bridging sciatic nerve defects in dogs, respectively, 8,10 cm defect length groups, each have six adult dogs, follow-up period of 12 months. Observation of animal limb function recovery, electromyographic changes and regeneration of nerve tissue manifestations, including HE staining, S100 immunohistochemistry, acetyl cholinesterase staining. [Results] to the length of the paragraphs in the nerve cells to the cell level, structural integrity and comprehensive evaluation of the residual myelin staining no difference. Vivo results showed that 8 cm acellular nerve graft group of dogs after 12 months of ankle joint can walk upright, while the 10 cm acellular nerve graft group of dogs 12 months of ankle joint can not walk upright. EMG examination showed that both groups of animals can be recorded in surgical limb motor and sensory evoked