Chinese and Western combined on the retinal ganglion cells in diabetic rats bcl2 and bax gene expression.docVIP

 PAGE \* MERGEFORMAT 12 Chinese and Western combined on the retinal ganglion cells in diabetic rats bcl2 and bax gene expression [Abstract] [Objective] To observe the treatment of diabetic rat retinal ganglion cells bcl 2 and bax gene expression. [Method] 40 male SD rats were randomly divided into control group, diabetic control group, metformin treatment groups, Chinese and Western medicine treatment group and combined group, small dose of intraperitoneal injection twice a streptozotocin-induced diabetic rat model. Chinese and Western medicine treatment group and combined treatment group 15 days before making model for preventive administration (in Chinese kidney Tongqiao side decoction gavage). Chinese and Western medicine group and the combination group after modeling give in the administration of metformin hydrochloride. into the mold after 6W dislocated rats were killed by adding liquid nitrogen, grinding out the eye , extracted RNA, the PCR gel electrophoresis, and then fluorescence PCR, detection of retinal ganglion cells bcl 2 and bax gene expression in the case. [results] in the combination group Western retinal ganglion cells bcl 2 and bax expression with other group with significant difference (P lt;0.05), was statistically significant. [Conclusion] Traditional Chinese medicine can affect the United retinal ganglion cells in diabetic rats bcl 2 and bax gene expression, thereby inhibiting the diabetic rat retinal ganglion apoptosis. [Keywords:] diabetic rats; ganglion cells; gene; in the Western Abstract: [Objective] To observe the effect of different treatments on bcl 2 and bax genetic expression of retinal ganglion cell in rats with diabetic retinopathy. [Methods] Forty male SD rats were randomly divided into 5 groups: normal group (N group), diabetic group (D group), metformin hydrochiloride treated group (MH group), Chinese medicine treated group (C group), Chinese western medicine combined treated group (CW group). D group was set up by