Chronic HBV infection in peripheral blood mononuclear cells expression of IFN- and its influencing factors.docVIP

 PAGE \* MERGEFORMAT 16 Chronic HBV infection in peripheral blood mononuclear cells expression of IFN- and its influencing factors Of: HONGKONG, Lin Shu-Mei, Zhang Shulin, Ye Feng, Zhang Xi, Zhao Yingren [Abstract] Objective To investigate the chronic hepatitis B virus (HBV) infection of interferon- (IFN-) expression and its influencing factors. Methods real-time quantitative RT-PCR, and ELISA were detected in peripheral blood of chronic HBV infection nuclear cells (PBMCs) of IFN- mRNA expression and serum levels of IFN- secretion, PBMCs were detected by nested PCR in HBV DNA. the result of chronic HBV infection and the expression of IFN- secretion was significantly lower than control group (P lt;0.01, P lt;0.05), chronic HBV carriers, IFN- expression and secretion were significantly lower than the HBeAg positive group (P all lt;0.05) and HBeAg negative group (P all lt;0.01), HBeAg positive group, IFN - expression and secretion were significantly lower than HBeAg negative group (P all lt;0.01), PBMCs of HBV DNA positive group and IFN- expression and secretion of HBV DNA were significantly lower than the negative group (P all lt;0.01), IFN- expression and secretion and serum HBV DNA levels were negatively correlated (P all lt;0.01). chronic HBV infection with low expression of IFN-, high viral load can inhibit the expression of IFN-. [Keywords:] Hepatitis B Hepatitis B virus DNA (HBV DNA) in peripheral blood mononuclear cells of interferon- (IFN-) ABSTRACT: Objective To study the expression of interferon- (IFN-) in peripheral blood monocytes (PBMCs) in patients with chronic HBV infection and factors that may affect its expression. Methods The expression of IFN- mRNA and secretion of IFN- in chronic HBV infectious patients were determined by real-time RT-PCR and ELISA, respectively; HBV DNA in PBMCs was detected by nested-PCR. Results The expression and secretion of IFN- were all significantly lower in chronic HBV infectious patients than in controls