Citrus medica L. var rDNA ITS sequencing and preliminary analysis of the characteristics of.docVIP

 PAGE \* MERGEFORMAT 17 Citrus medica L. var rDNA ITS sequencing and preliminary analysis of the characteristics of Author: Gao Xiaoxia, Chen Xiaoying, Luoyuan Health 【Key Words】 bergamot;,, citron;,, rDNA, ITS sequences Abstract: Objective To analyze the wide bergamot and its related species citron of rDNA ITS sequences and its laws. Methods Direct sequencing of PCR was measured by wide bergamot and citron rDNA ITS sequences for sequence variation analysis. The results of four plant samples rDNA ITS sequence length of 644 ~ 672 bp. In which 5.8S rDNA length of 165 bp, coding region more conservative. Canton bergamot and citron ITS1 and ITS2 sequences of the average genetic distance of 0.009 5 and 0.014, respectively 2. Conclusion rDNA ITS sequences can provide molecular identification of bergamot reference system. Keywords:: Bergamot; citron; rDNA ITS sequences Abstract: ObjectiveThe ribosomal DNA internao transcribed spacers (ITS) and 5.8S rDNA ITS sequence of Citrus medica L.var.sarcodactylis (Nootc). Swingle and C.medicaL. Were sequenced.MethodsThe rDNA ITS gene fragment was amplified by PCR with universal primers of rDNA ITS and sequenced.ResultsThe DNA sequence of ITS ranged from 644 bp to 672 bp and the 5.8S gene of all of the samples was 165 bp in size.Distance values of ITS1 and ITS2 sequence, estimated according to Kimura two-parameter models between Citrus medicaL.var.sarcodactylis (Noot.) Swingle and its adulterant and C.medica L., were 0.0095 and 0.0142, respectively.ConclusionrDNA ITS sequence may be the evidence for the molecular authentication of Citrus medica L.var.sarcodactylis (Noot .) Swingle. Keywords:: Citrus medica L.var.sarcodactyis (Noot.) Swingle; C.medica L.; rDNA internal transcribed spacers sequence DNA molecular marker technology into the identification and quality evaluation of Chinese herbal medicine research areas, providing a large number of potential characteristics of the Chinese