CMV-specific IgG on the detection of antibodies and its application.docVIP

 PAGE \* MERGEFORMAT 30 CMV-specific IgG on the detection of antibodies and its application OF: Lisa Hou Lin Zhang Mengan cattle to the Blue Valley School Pu Ying Ho Huan Xu Ma [Abstract] Objective To establish a rapid, simple and practical for women of childbearing age in serum cytomegalovirus (CMV) IgG antibody screening methods, and explored its application. Methods recombinant CMV (pp150 +52) antigen ; to the National Population and Family Planning Commission of Science and Technology Research Institute (referred to as the by) the establishment of the recombinant CMV (pp150 +52) antigen as the coating antigen, 700 IU / L as the critical value, with the indirect ELISA method, Shenzhen Baoan District 1 024 cases of CMV IgG antibodies in serum samples for testing. Results The sensitivity is 13 IU / L, coefficient of variation of 4.7% to 7.0% and between 7.6% coefficient of variation of 14.4%, and with SORIN kit produced compared 2 methods were the most correlation, Kappa 0.82; Shenzhen Baoan District 1 024 serum samples positive for CMV IgG antibody was 93.3% (955 / 1 024), negative rate was 6.7% (69 / 1 024) . Conclusion The established ELISA method is fast, simple and practical, can be used in women of childbearing age CMV  IgG antibody screening. [Keywords:] cytomegalovirus; IgG antibodies; ELISA; screening; women of childbearing age [Abstract] Objective To establish a rapid, serum and convenient method for the screening of cytomegalovirus (CMV) specific IgG antibody in sera samples of women of childbearing age, and to evaluate the preliminary significance of application.MethodsThe measurement of CMV IgG was conducted by indirect enzyme linked immunosorbent assay using recombinant CMV Ag (pp150 +52) as coating antigen, and 700 IU / L as Cut off value. ResultsThe specific CMV IgG antibodies in sera were detected in 1 024 women of childbearing age in Shenzhen, by using the method established by our study and the ELISA assay kid provided by Diasor