Coarse roots Rubus polysaccharide determination.docVIP

 PAGE \* MERGEFORMAT 12 Coarse roots Rubus polysaccharide determination Author: Zhou Heng, Li Tianjiao, Hong Zhenfeng, Hu Juan 【Abstract】 Objective To establish the coarse roots Rubus method for the determination of polysaccharide. Methods phenol - sulfuric acid method color, the standard curve in the 490 nm light punishment Determination of crude polysaccharide content of the roots of Rubus. The results of the regression equation: Y = 0.8325X 0.022 3, r = 0.999 8, the measured coarse roots Rubus polysaccharide content 9.473%, the average recovery was 99.69%, RSD = 0.56% (n = 6). Conclusion The method is simple, precision and reproducibility are good, can be used for rough Rubus extraction and determination of polysaccharides. Keywords: rough Rubus; polysaccharide; phenol - sulfuric acid method; spectrophotometry Abstract: ObjectiveTo study the optimum extration process of polysaccharides from Rubus alceaefolius Poir. MethodsThe content of polysaccharids from Rubus alceaefolius Poir was determined by spectrophotometry at 490 nm. ResultsThe regression equation was Y = 0.832 5 × 0.022 3, r = 0.999 8. The percent of polysaccharide in Rubus alceaefolius Poir was 9.473%. The average recovery rate was 99.69% and RSD was 0.56% (n = 6). ConclusionThe method is proved to be simple, accurate and reliable, and it can be used to extract and determine the polysaccharides from Rubus alceaefolius Poir. Keywords:: Rubus alceaefolius Poir; Polysacharide; Determination Coarse leaves of dicotyledonous plants of Rubus Rosaceae, Rubus coarse roots, leaves can be used as medicine, with blood circulation Huayu, heat the efficacy of detoxification [1], can Kiyotoshi hot and humid, and so hot and humid due to intrinsic toxic silt removal. According to the Guangzhou military to prepare a “common herbs Manual” is written: “blood circulation Removes silt, heat to stop bleeding, cure acute and chronic hepatitis, hepatosplenomegaly, march hemoglobi