Compound Tianqi Yi zhi extraction and quality standard.docVIP

 PAGE \* MERGEFORMAT 14 Compound Tianqi Yi zhi extraction and quality standard [Abstract] Objective To optimize the best combination Tianqi Yi zhi extraction process, and establish quality standards. Method to rate as the index of total saponins, the use of orthogonal test compound Tianqi Yi zhi optimum extraction. With TLC method of preparation notoginseng, Panax ginseng qualitative identification, preparations were determined by spectrophotometry of total saponin content. Results optimum extraction conditions were: microwave extraction 3 times, 10 times the amount of water, each time for 30 min.TLC method chromatography with reference substance for the test product was the same color as the corresponding position of the spots, and negative non-interference. ginsenoside Re in the range of 40 ~ 200 μg good linear relationship, Y = 250.83X-0.017, r = 0.997 2, precision and recovery meet the requirements. Conclusion The trial to determine the extraction process and quality standards in production feasible. [Keywords:] compound Tianqi Yi zhi, orthogonal, extraction technology, quality standard, TLC, HPLC Abstract: Objective To investigate the best conditions of extraction techniques for compound Tianqi Yizhi granule and to establish its quality standards. Methods The best conditions of extraction techniques for compound Tianqi Yizhi granule were optimized by orthogonal experiments, with the yield of total saponins as the index . The qualitative analysis of Panax Notoginseng and Ginseng with Thin-Layer Chromatography and determination of total saponins with UV-spectrophotometry was made. Results The optimal condition of total saponins was that the microwave extraction for 3 times and extracting for 30 minutes, the material- water rate of 1:10. In TLC procedure, the control material and the test sample showed the same colored spots, while the negative control sample showed no interference. Ginsenoside Re showed a good linear relationship at the range of 40