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PAGE \* MERGEFORMAT 4
Compound Sijunzitang affect the function of mouse anti-diarrhea preliminary study
[Abstract] In this study, based on TCM syndrome differentiation based on the theory of in vitro inhibitory experiment (Plate paper method), observation of compound Sijunzitang with commonly used antibiotics (norfloxacin, cephalexin) and antibacterial effects, and experiments in vivo antibacterial (ig E. coli), observed in mice of Kunming species of diarrhea cases within 10 hours to study the antibacterial compound Sijunzitang effect and mechanism to determine compound Sijunzitang as feed additives to enhance the possibility of the role of animal anti-diarrhea.
[Keywords:] compound Sijunzitang mouse anti-diarrhea feed additive
Diarrhea is a farming more difficult problem, aquaculture cause great harm. At present, Chinese herbal medicine at home and abroad for animal feed additives to promote growth and improve the reproductive capacity has done a lot of research, but its in the anti-bacterial disease prevention research are not many. Many scholars at home and abroad on the application of this herbal animal feed additive inhibition diarrhea had a lot of research, but so far, many experiments in this regard only in vivo or in vitro, the present study two kinds of experimental methods to study the synthesis of compound 4 as a gentleman’s Soup The mechanism of anti-diarrhea, feed additives and advantages.
1 Materials and methods
1.1 Experimental Materials
Animal: 70 Kunming mice, weighing 16 ~ 18g, male and female in half, were purchased in Nanjing Medical University Animal Experiment Center.
Experimental drugs: prescriptions compound Sijunzitang, rhubarb (from Nanjing Agricultural University in pharmacies), E. coli stock solution (from Nanjing Agricultural University Infectious Diseases Section of Animal Sciences), eosin methylene blue reagent, norfloxacin capsules, cephalexin capsules .
Experimental equipment: high-pressure sterilization pot, ster
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