Curcumin inhibits cancer cell proliferation and induced apoptosis in vitro analysis.docVIP

 PAGE \* MERGEFORMAT 10 Curcumin inhibits cancer cell proliferation and induced apoptosis in vitro analysis [Abstract] Objective: To study the traditional Chinese medicine curcumin (Cur) SiHa cervical cancer cell proliferation inhibition and induction of apoptosis. METHODS: Various concentrations of curcumin (10 umol / L, 20 umol / L, 30 umol / L), sub 12h, 24h, 48h, 72h 4 time points in treatment SiHa cells, changes in cell morphology observed by light microscopy; with tetrazolium blue (MTT) cell proliferation inhibition rate method; by transmission electron microscopy and agarose gel electrophoresis apoptosis. Results: Curcumin inhibited the proliferation of SiHa cells, the effects were significant time and dose-effect relationship, the difference was statistically significant (P lt;0.01); curcumin induced apoptosis in SiHa cells , transmission electron microscopy of apoptotic cells; agarose gel electrophoresis apoptosis typical DNA “ladder” bands. CONCLUSION: SiHa cells with curcumin in vitro proliferation and promote apoptosis. [Keywords:] curcumin; SiHa cells; apoptosis; agarose gel electrophoresis Cervical cancer is a common gynecological malignancies in recent years have increased incidence and younger trend. The current treatment of cervical cancer is still surgery, but radical surgery of the lower 5-year survival rate, cervical cancer sensitivity to radiotherapy also have individual differences, and local radiotherapy prone to metastasis and recurrence. So look for an effective cervical cancer chemotherapeutic drugs is of great significance. In this paper, traditional Chinese medicine curcumin on human cervical cancer SiHa cell proliferation and apoptosis, as find new and effective drug treatment of cervical cancer provides a theoretical basis. Curcumin is extracted from the ginger family plant a food coloring, with a wide range of pharmacological effects, such as anti-inflammatory, antioxidant, anticoagulant, lipid-lowering and anti-athero