Cytoskeleton in high-mobility group protein B1-induced human umbilical vein endothelial cell expressed and secreted in the role of IL6.docVIP

 PAGE \* MERGEFORMAT 13 Cytoskeleton in high-mobility group protein B1-induced human umbilical vein endothelial cell expressed and secreted in the role of IL6 Author: Jia Xu Yang Li Zhi-Jie Liu Jinghua DENG Peng Yong [Abstract] Objective: To observe the in vitro reconstitution of high mobility group protein B1 (HMGB1)-induced human umbilical vein endothelial cells (HUVEC) secretion of the release of interleukin -6 (IL  6) of the dose-effect relationship to explore the cytoskeleton in the HMGB1-induced HUVEC the release of IL  6 in the role. Methods: To construct prokaryotic expression plasmid and expression and purification of His  EGFP  HMGB1 and His  EGFP fusion protein to stimulate the in vitro cultured HUVEC; the use of liquid-chip analysis of systematic observations of HMGB1 and the associated cytoskeleton inhibitors or low-temperature cultured cells secrete IL under  6 factor situation. Results: The digestion and DNA sequencing to prove that the prokaryotic expression vector is correct, expression and purification of the recombinant protein by SDS  PAGE electrophoretic analysis is the correct size to stimulate the in vitro HUVEC after the high expression of IL  6 and has a clear dose-response relationship; Nocodazole, Cytochalasin D and other cytoskeleton inhibitors and low-temperature culture significantly inhibited after expression of IL  6. Conclusion: In vitro recombinant human HMGB1 protein can effectively induce HUVEC secretion and release of IL  6, while the structure of the cytoskeleton play an important role in this process, and has a clear energy dependence. [Keywords:] high mobility group protein cytoskeleton of vascular endothelial human umbilical vein interleukin-6 [Abstract] Objective: To understand the role of cytoskeleton in the expression of interleukin  6 (IL  6) in human umbilical vein endothelial cells (HUVECs) under the stimulation of the fusion HMGB1 protein. Methods: The coding sequence of HMGB1 was ampli