Dehydrogenation of liquid acid content in foods by HPLC analysis.docVIP

 PAGE \* MERGEFORMAT 8 Dehydrogenation of liquid acid content in foods by HPLC analysis [Keywords:] acetic acid dehydrogenation of liquid foods by high performance liquid chromatographic method Dehydrogenation acid (DHA, Dehydroacetic), alias diacetyl acetoacetate, solid white or light yellow crystalline powder, odorless, tasteless, melting point 108 ~ 110 ℃, boiling point 270 ℃, is a low toxicity and high anti-corrosion, anti-mildew agent. in acid, alkaline conditions have some antibacterial effect, especially the strongest inhibition of mold. China since the mid 20th century, 70 so far for food preservatives has been used for fruit juice, pickles, fermented bean curd, margarine , lactic acid bacteria drinks, jam and other food preservatives [1]. Although its antimicrobial activity is effective, but its high toxicity has limited the dehydrogenation of the shortcomings of the extensive application of acetic acid. dehydrogenation acid is absorbed quickly by the body , into human plasma and dispersed after a number of organs, there are a variety of oxidase inhibitory effect in vivo. At present, the security of acetate dehydrogenation been questioned, the international community has explicitly prohibited the use of within a certain range to allow its use , but the maximum amount allowed 0.3g/kg [2]. Acid Dehydrogenation of the current national standard test method for gas chromatography [3], sample pre-treatment process involves organic solvent extraction, concentration and other steps to operate more trouble, but is also likely to cause dehydrogenation acid chromatographic peak tailing, making the difficult to obtain accurate qualitative and quantitative results [4]. in food by high performance liquid chromatography method for dehydrogenation of acid have been reported, such as the use of ultrasonic extraction, such as sodium hydroxide solution, extracted moon cake soaked in acid after dehydrogenation then injected into the column for separation and determ