Dengzhanhua extracts in vitro cultured retinal ganglion cells in high gene expression of apoptosis.docVIP

 PAGE \* MERGEFORMAT 9 Dengzhanhua extracts in vitro cultured retinal ganglion cells in high gene expression of apoptosis [Abstract] Objective To observe the Dengzhanhua extract (DSX) High in vitro cultured retinal ganglion cell apoptosis and apoptosis gene expression. Methods (1) in RGCs adding DSX, pressurized cultured cells were collected, with the percentage of apoptotic cells detected by flow cytometry. (2) in RGCs adding DSX, pressurized cultured cells were collected, using gene chips to find apoptotic gene expression. Results (1) in the high-pressure RGCs cultured, DSX group the percentage of apoptosis than the model group and control group. (2) under conditions of high culture with the neuronal apoptosis-related genes S100B expression returned to normal, NGFR expression down. Conclusion DSX is a protective effect of RGCs effective monomer, enabling high expression in cultured RGCs of S100B return to normal, NGFR expression down. [Keywords:] retinal ganglion cells; cell culture; pressure; cell apoptosis Apoptotic genetic expression of RGCs by bolting constituent from Herba Erigerontis cultured in vitro by high pressure [Abstract] Objective To observe the apoptosis and look for apoptotic gene of RGCs cultured in vitro by high pressure by adding the constituent come from Herba Erigerontis (DSX). Methods We build rat retinal cells and RGCs cultured in virto by high pressure.Put 80 mmHg pressure to RGCs in close tightly culture  bottle for four hours, then collect RGCs and check the sum of apoptosis cells by flow cytometry (FCM). Put the pressure 80 mmHg to RGCs in close tightly culture  bottle for four hours, then collect RGCs and look for the apoptotic gene by gene chip. Results The apoptosis of model group and control group higher than DSX group cultured in the pressure of 80 mmHg.Two apoptotic gene (S100B, NGFR) express up  regulation. Conclusion DSX was the most effective constituent in protecting RGCs in vitro.The expressing up  regul