Determination of Aralia extract the content of oleanolic acid.docVIP

 PAGE \* MERGEFORMAT 9 Determination of Aralia extract the content of oleanolic acid Of: Tian Ji, Li Xiang wings, Bing, Xiao Shunhan [Abstract] Objective To extract Aralia and determination of oleanolic acid. Methods using high performance liquid chromatography (HPLC method, column was Kromasil C18 column (250 mm 4.6 mm, 5 m), mobile phase methanol - water (95:5, detection wavelength 210 nm, column temperature 30 , the flow rate 1.0 ml min-1, the injection volume was 10l. Results of oleanolic acid in the range of 0.502 ~ 4.016g good with the peak area linear relationship (r = 0.999 9, the average recovery rate of 96.38%, RSD 1.17%. Conclusion The method is sensitive, accurate, and can be used to extract Aralia and quality control. [Keywords:] elata, extract, oleanolic acid, determination, HPLC Abstract: ObjectiveTo establish a method for the determination of oleanolic acid in root bark of Aralia and its extracts. MethodsA Kromasil C18 column (250mm 4.6mm, 5m) with mobile phase consisting of methanol-water (95:5) was adopted, and the detection wavelength was set at 210 nm. The column temperature was 30 . The flow rate was 1.0 ml min-1, and the sample volume was 10l. ResultsThe calibration curve showed good linearity over the range of 0.502 ~ 4.016g (r = 0.999 9.The average recovery was 96.38% with RSD 1.17%. ConclusionThe method is sensitive, accurate, and is suitable for the quality control of root bark of Aralia and its extracts. Keywords:: Root bark of Aralia, Extracts, Oleanolic acid, Content determination, HPLC Aralia, Aralia, also known as white as the genus of Araliaceae Aralia elata root bark of Aralia chinensis L. with rheumatism, Lee urine, loose congestion, swelling and drug efficacy [1]. All over the country to variety of species of Aralia elata root bark for medicine, a common elata, Aralia taibaiensis, Aralia elata, Aralia Rattus other species. elata is the major active ingredient of plant Aralia saponins, aglycone as olea