Determination of mycophenolic acid in renal transplant recipients early plasma concentrations of.docVIP

 PAGE \* MERGEFORMAT 12 Determination of mycophenolic acid in renal transplant recipients early plasma concentrations of Of: Dong Weihua, Dong Yalin, Zheng Xin, You Haisheng [Abstract] Objective To establish a HPLC method for the determination of mycophenolic acid in human plasma concentration of simple methods. Method using high performance liquid chromatography column was C18 column (3.9mm 300mm), to 40mmoL / L tetrabutylammonium bromide in acetonitrile [55:45 (V: V), H3PO4 adjust pH = 4] as the mobile phase, flow rate 0.8mL/min, UV detection wavelength of 254nm, protein precipitation method with methanol blood processing. mycophenolic acid results The linear range was 0.8 ~ 51.54mg / L (r = 0.9998), average relative recovery rate 95.5% 109.2% extraction recovery of “90%, inter-and intra RSD lt;10%. Conclusion The method is accurate, simple, mycophenolic acid can be used for therapeutic drug monitoring and bio-equivalence study. [Keywords:] kidney transplantation, mycophenolate mofetil, mycophenolic acid, high performance liquid chromatography (HPLC), plasma concentration ABSTRACT: Objective To establish a high performance liquid chromatography method for determination of mycophenolic acid in human plasma. Methods The method was performed with C18 column (3.9mm 300mm). The mobile phase consisted of 40mmoL / L TBA solution acetonitrile adjusted to pH 4 with orthophosphoric acid (55:45, V: V), with a flow rate 0.8mL/min. The UV detection wavelength was 254nm. Plasma samples were extracted with methanol. Results The linear concentration of the calibration curve ranged from 0.8 to 51.45mg / L (r = 0.9998). The relative recoveries were between 95.5% and 109.2%, and the extraction recoveries were more than 90%. Relative standard deviation (RSD) of intraday and interday assays were both less than 10%. Conclusion The method is proved to be reliable and simple for monitoring mycophenolic acid in human plasma and bioequivalence research. KEY WORDS: ren