Determination of particle heat-toxin levels of chlorogenic acid.docVIP

 PAGE \* MERGEFORMAT 8 Determination of particle heat-toxin levels of chlorogenic acid Of: Li Ping, Takamatsu, Chen Yujie, high-shan [Abstract] Objective Determination of the establishment of heat-toxin levels of chlorogenic acid particles. Methods column was YMC ODS-A column Corporation (150 mm × 4.6 mm, 5 μm), mobile phase of acetonitrile - 0.2% phosphoric acid (10:90), flow rate 1.0 mL / min, detection wavelength was 327 nm. the results of the detection of chlorogenic acid concentration in the range of 0.084 8 ~ 0.848 μg and peak area was linear (r = 0.999 9), the average recovery was 102.60%, RSD = 1.69% (n = 6). Conclusion The method is simple, rapid, and can be used to heat the quality control of drug levels. [Keywords:] hot drugs even grain, chlorogenic acid, HPLC, determination Abstract: Objective To establish an HPLC method for the determination of Chlorogenic acid in Reduping Keli. Methods The determination was performed by HPLC using an YMC ODS-A column (150 mm × 4.6 mm, 5 μm), with mobile phase consisted of acetonitrile- 0.2% H3PO4 solution (10:90) at a flow rate of 1.0 mL / min and a detection wavelength of 327 nm. Results The calibration curve for Chlorogenic acid was linear in the concentration range of 0.008 5 ~ 0.084 8 mg / mL (r = 0.999 9). The average recovery was 102.60% (RSD = 1.69%, n = 6). Conclusion The method is simple, rapid and applicable for the quality control of Reduping Keli. Keywords:: Reduping Keli, Chlorogenic acid, HPLC, content determination Hot particles from the honeysuckle virus levels, gypsum, Scrophulariaceae, to yellow, and other traditional Chinese medicine made of the effectiveness of a detoxification for the treatment of influenza, upper respiratory tract infection and a variety of heat embolism [1]. Chlorogenic acid in the preparation main drugs of the main active ingredients of honeysuckle, with a significant anti-inflammatory, bactericidal, antiviral effect [2]. I by high performance liquid chrom