Different concentrations of dexamethasone on human bone marrow mesenchymal stem cell biological characteristics of.docVIP

 PAGE \* MERGEFORMAT 18 Different concentrations of dexamethasone on human bone marrow mesenchymal stem cell biological characteristics of Of: Pangjin Hui, Huang Huang Yuan, Zhang Quan, Ji Bin, Cao Chengfu [Abstract] Objective To observe the different concentrations of dexamethasone on cultured human bone marrow mesenchymal stem cells (hMSCs) into osteoblasts, proliferation and apoptosis. Methods to obtain the density gradient separation of human bone marrow mesenchymal stem cells in vitro culture to 1 10-9,1 10-8,1 10-7,1 10-6mol / L 4 different concentrations of Dex treated cells. was detected by cell alkaline phosphatase (ALP activity, RT PCR detection of osteopontin (OPN) gene expression, methyl thiazolyl tetrazolium (MTT colorimetric assay cell proliferation rate in each group, the apoptosis rate by flow cytometry. The results of low concentrations ( 1 10-9mol / L) of dexamethasone on the expression of ALP no significant effect (Pgt; 0.05), and 1 10-8mol / L and higher concentrations of dexamethasone significantly increased the ALP activity (P lt;0.01) All experimental groups were OPN mRNA expression in hMSCs, and its expression intensity increased with increased concentrations of dexamethasone, 1 10-9mol / L dexamethasone had no effect on cell proliferation (Pgt; 0.05), 1 10 - 8mol / L and higher concentrations of dexamethasone can inhibit cell proliferation (P lt;0.01), in vitro apoptosis rate anywhere hMSCs increased with increasing concentrations of dexamethasone (P lt;0.01). Conclusion 1 10-8mol / L and higher concentrations of dexamethasone can significantly enhance the ability of hMSCs into osteoblasts, while inhibiting cell proliferation and promote apoptosis. [Keywords:] mesenchymal stem cells, dexamethasone, alkaline phosphatase, osteopontin, cell proliferation, apoptosis Abstract: Objective To observe the effect of dexamethasone on the biological characteristics of human mesenchymal stem cells (hMSCs) derived from bone marro