Different concentrations of organic iodine in human thyroid cell proliferation.docVIP

 PAGE \* MERGEFORMAT 4 Different concentrations of organic iodine in human thyroid cell proliferation Authors: Zhao Yan, Lin Xin Ying, Guo Dongmei, Wang Shue, Liu Xiaoting [Keywords:] organic iodine (DIT); thyroid cells (TEC); proliferation Abstract: Objective To study the different concentrations of organic iodine (DIT) on cultured human thyroid cells (TEC) proliferation effects, and and inorganic iodine (KI) compared to explore a more safe and effective iodine preparation. Methods thyroid adenoma and normal thyroid tissue adjacent to the application of primary cell culture technology, cell culture, with different concentrations (10-8 ~ 10-3mol / L) of the DIT, and KI to stimulate the thyroid cells in monolayer culture, using 4-methyl thiazolyl tetrazolium (MTT) Determination of each group of thyroid cell proliferation. The results of the low concentration of KI did not affect the growth of human thyroid cells, while the high concentration of KI has inhibitory effect on cell growth, and a dose - time-dependent relationship; with the control group, the higher the concentration, the longer the suppression more obvious. DIT Group is only at high concentrations Caidui thyroid cells were inhibited, and the DIT each dose group and KI each dose group at the same dose levels compared to 22 found that, DIT pairs of cells in vitro significantly lighter than KI cells in vivo. Conclusions high concentrations of iodine can inhibit thyroid cell growth, DIT on cell growth inhibition than KI, namely, the cell damage DIT lighter than the KI. Keywords:: organic iodine (DIT); thyroid cells (TEC); proliferation Effect of DIT on proliferation of human thyrocytes in vitro Abstract: Objective To investigate the influence of DIT on the proliferation of human thyrocytes and compare DIT with KI.To study a good kind of supplement to USI that was safe and effective.Methods The human thyroid epithelium cells from para-adenoma normal tissues from thyroid adenoma patients were culture