Different methods of separation of human umbilical cord blood hematopoietic cells Comparative Study.docVIP

 PAGE \* MERGEFORMAT 5 Different methods of separation of human umbilical cord blood hematopoietic cells Comparative Study [Abstract] Objective: To compare the different methods of separation of human umbilical cord blood hematopoietic cells recovery rate. Methods: using methyl cellulose, gelatin, hydroxyethyl starch, red blood cell lysate isolated cord blood stem cells to calculate recovery of nucleated cells. Giemsa staining calculate the percentage of mononuclear cells. Results: The four kinds of methods after the separation recoveries were 66.13%, 74.91%, 66.13%, 62.90%. Conclusion: The four kinds of methods effects are good, of which gelatin separate the best results. [Keywords:] human umbilical cord blood hematopoietic cells in methyl cellulose gelatin separation of red blood cell lysis buffer Cord blood is rich in hematopoietic stem cells in hematopoietic stem cell transplantation, in addition to bone marrow, peripheral blood hematopoietic stem cells outside the ideal source. Umbilical cord blood collection convenient source of wealth. Studies have shown that cord blood mononuclear cell proliferation, the cellular immune activity is still lower than adult peripheral blood [1]. We use different methods of separation of umbilical cord blood hematopoietic cells and compare their recoveries with a view to find the best method of separation of hematopoietic cells. 1 Materials and methods 1.1 Materials blood samples from Changchun City Maternity Hospital. Requirements: No acute or chronic infections and blood system diseases, non-high-risk pregnancy, maternal, full-term natural delivery. After the cord blood collection, 4 ℃ preservation, not more than 24 h. Methyl cellulose, gelatin Sigma separation. Hydroxyethyl starch Sigma imported. 1.2 Methods (1) 6 copies of umbilical cord blood an average of 70 ~ 80 mL. Take cord blood 40 mL, the average is divided into four equal parts, each 10 mL. Calculating the total nucleated cells (TNC) number. (2) taking a