Diammonium glycyrrhizinate injection on renal ischemia - reperfusion injury in rats.docVIP

 PAGE \* MERGEFORMAT 5 Diammonium glycyrrhizinate injection on renal ischemia / reperfusion injury in rats [Abstract] Objective To investigate the effect of diammonium glycyrrhizinate (DG) on renal ischemia / reperfusion (ischemia / reperfusion, I / R) protective effect. Methods The right kidney removed, after 60 min occlusion of left renal artery to restore method of reperfusion in vivo replication of renal I / R injury model, occlusion of artery 30 min before the intravenous injection of DG 15 mg / kg, or DG 30 mg / kg . Detection of reperfusion 1 h and reperfusion 24 h, blood urea nitrogen (BUN), serum creatinine (Scr), and tumor necrosis factor-α (TNF-α ) and interleukin -1 (IL-1) level and reperfusion 24 h, renal tissue malondialdehyde (MDA) and superoxide dismutase (SOD) content. The results of DG can significantly reduce I / R caused the increase of serum BUN and Scr, reducing TNF-α and IL-1 generation, improve the renal tissue SOD activity, reduced MDA formation. Conclusion DG of renal I / R significantly protective effect, the mechanism with the DG to reduce lipid peroxidation, reducing cytokine TNF-α and IL-1 level in the. [Keywords:] glycyrrhizic acid, diammonium renal ischemia-reperfusion injury cytokine Abstract: ObjectiveTo study the protective effect of diammonium glycyrrhizinate injection (DG) on renal ischemia / reperfusion (I / R) in rats.MethodsA model of renal I / R was made by clamping double renal pedical for 60 min and reperfusion for 24 h. The injection of DG (15mg/kg or 30mg/kg) was pretreated before renal ischemia. The leveles of serum urea nitrogen (BUN), serum creatinine (Scr), tumor necrosis factor-α (TNF-α ), interleukin-1 ( IL-1) in serum were detected after reperfusion for 1 h and 24 h respectively, and the content of MDA and SOD in renal tissuses were measured after reperfusion for 24 h.ResultsDG significantly decreased the level of BUN and Scr, decreased the concentration of TNF-α and IL-1 in serum. Pretreatment with DG