Diffusa on cervical cancer Hela cell cycle apoptosis and telomerase activity.docVIP

 PAGE \* MERGEFORMAT 12 Diffusa on cervical cancer Hela cell cycle apoptosis and telomerase activity Of: superb, Liu Ying, Cai Xiaomin, Hao Xingzhi [Abstract] Objective To study the traditional Chinese medicine diffusa (Hedyotic diffusa, HD on telomerase activity in human cervical carcinoma Hela cells, cell cycle and apoptosis, to investigate the inhibition of Hela cells in HD tumor activity and telomerase. Methods concentration of each of the different role of Hela cells in vitro, using PCR-TRAP-ELISA Quantitative Detection of HD treatment Hela cells telomerase activity level, the synchronization of cell cycle detected by flow cytometry and the apoptosis rate of change . Results Hela cells were positive for telomerase, the role of a certain concentration of HD after a certain time in Hela cells can Hela cells were arrested in S phase and induce apoptosis, while telomerase activity was significantly reduced. Conclusions may be adopted HD Hela cell cycle changes (S phase arrest and also induced apoptosis, decreased telomerase activity to achieve anti-tumor effect. [Keywords:] diffusa, cervical cancer Hela cells, cell cycle, apoptosis, telomerase In recent years some scholars diffusa (Hedyotic diffusa, HD anti-tumor effects have been studied, its essential to be sure that anti-tumor effect [1-4], but its mechanism of action, especially not in-depth understanding of the molecular biological mechanisms. Telomerase expression and apoptosis in tumors and play an important role in the development, how to induce tumor cell apoptosis and regulation of telomerase activity in cancer treatment has become a new way. HD vitro and in vivo has obvious tumor effect, whether this effect induced tumor cell apoptosis, cell cycle arrest and inhibition of telomerase activity, not more than reported in literature. In this study, human cervical carcinoma Hela cells were studied, using PCR products of polypropylene amide gel electrophoresis and silver staining qualitative d