Diffuse axonal injury in experimental apparatus and the animal model.docVIP

 PAGE \* MERGEFORMAT 15 Diffuse axonal injury in experimental apparatus and the animal model Of: CHANG Songjin Ning, Chen Jingyu, Zhang Fenru, Shou-Xun Liu [Abstract] A purpose-made diffuse axonal injury (DAI) DAI device and successfully established animal model. Methods instant self-rotating head injury unit, the rat head in an instant (lt;3 ms) rotated 90 causing shear damage . observed in rats after injury and behavioral changes in vital signs, 2,6,12,24,36,72 h after injury and the injury of 10 d animals were killed to prepare brain paraffin sections and HE staining silver line, neuraxial light microscope. Results appear immediately after injury, rats were the primary coma, including two within 20 min after injury deaths, more than the duration ranging from 1-30 min after injury in rats breathing rhythm disturbance pupillary light reflex reduced or lost, wake up after the reaction have varying degrees of decline, physical activities, retardation, widely visible subarachnoid hemorrhage or intraventricular hemorrhage, light microscope, leather cord junction, corpus callosum, brainstem , small white matter axonal nerve and other parts have different degrees of swelling, fracture, axonal ball formation, the late microglial cell proliferation, was nest-like aggregation of local. Conclusion The device can cause brain DAI, and has a simple, controllable, the exact characteristics, suitable for medium and small animal models of DAI Study. [Keywords:] diffuse axonal injury in rats animal model experimental apparatus ABSTRACT: Objective To design a new experimental facility to induce rat diffuse axonal injury model. Methods Rat diffuse axonal injury was induced by a new experimental facility, which was developed to let the rat head spin 90 degree at the moment to cause shearing injury. Vital sign and behavior of rat were measured simultaneously. The rats were sacrificed at 2 h, 6 h, 12 h, 24 h, 36 h, 72 h and 10 d after injury, respectively, and the brai