Digoxigenin labeled probe in the DNA of recombinant human interferon 2b Residues.docVIP

 PAGE \* MERGEFORMAT 9 Digoxigenin labeled probe in the DNA of recombinant human interferon 2b Residues [Abstract] Abstract: Objective To establish a test injection of recombinant human interferon @ 2b semi residues of exogenous DNA. Methods for recombinant human interferon @ 2b engineering strain genomic DNA as a template, digoxigenin labeled probe, as hybridization and the color reaction. Results per cent in semi-dose exogenous DNA residues were less than 10 ng. Conclusions The detection specificity stronger than the safe and easy operation, can be used for recombinant human interferon @ 2 b during the preparation of quality control and verification of semi-finished products. [Keywords:] DIG DNA residues of recombinant human interferon @ 2b Injection of recombinant human interferon @ 2b is the use of carrying human leukocyte interferon @ 2 b gene of E. coli producing the recombinant plasmid obtained, with broad-spectrum antiviral, inhibits cell proliferation and enhance immune function and so on. Primarily for the treatment of certain viral diseases such as acute and chronic viral hepatitis, herpes zoster, warts and so on. As used in the production process engineering bacteria E. coli, and the residual host cell DNA recombinant drugs is the potential carcinogenicity of specific impurities, so the interference Su @ 2 b semi-finished products of exogenous DNA is extremely important residues [1,2]. This article uses digoxigenin labeled probe hybridization assay for detecting injection of recombinant human interferon @ 2 b semi-finished products in the residual exogenous DNA operation more safe and easy, can be used for recombinant human interferon @ 2 b during the preparation of quality control to ensure its security in line with relevant quality control criteria [3]. 1 Materials and methods 1.1 Materials and reagents Recombinant human interferon @ 2b engineering bacteria host strain (Beijing Institute of Health and semi-finished products of