DNR co arsenic trioxide on K562-A02 cell line.docVIP

 PAGE \* MERGEFORMAT 14 DNR co arsenic trioxide on K562/A02 cell line Of: Ke, Jia-Hua Ding, Wu Weiwei, Bao-An Chen, Gu Yan, Yuan Peng [Abstract] Objective: To study the arsenic trioxide (As2O3) and daunorubicin (DNR, after combined application of human leukemia cell line resistant to the role of K562/A02 to explore the possibility of clinical application. Methods: The MTT colorimetric assay DNR DNR alone and in combination with different concentrations of As2O3 the growth inhibitory effect detected by flow cytometry intracellular DNR concentration and apoptosis. Results: DNR half of K562/A02 cell inhibitory concentration (IC50 = 15.4 mg / L, plus 0.25,0.5,1.0 mol / L As2O3 when the IC50 were 1.73,0.78,1.42 mg/L.1 mg / L DNR role in K562/A02 48 h after the apoptosis rate was (17.4 + -2.19)% , 0.5 mol / L As2O3 role in K562/A02 48 h after the apoptosis rate was (53.8 + -5.6)%, while the combination of two drugs apoptosis rate was (65.5 + -6.2)%, but the combination of DNR after intracellular concentration did not increase. Conclusion: The low concentration of As2O3 in combination with the DNR on the inhibition K562/A02 a synergistic effect, its effect and the increase in drugs related to induction of apoptosis, rather than by increasing the concentration of intracellular DNR. [Keywords:] Drug Therapy, Combination, multidrug resistance, arsenic trioxide, daunorubicin [Abstract] Objective: To explore the cytotoxity of combination of arsenic trioxide and daunorubicin on multidrug resistance of cell line K562/A02, and study the feasibility of combination of two drugs in clinical drug therapy.Methods: MTT assay were used to measure the growth inhibitory effect of DNR with or without various concentration of As2O3. The intracellular concentration of DNR and apoptotic changes were observed by flow cytometry. Results: The IC50 of DNR on K562/A02 cells was 15.4 mg / L. The IC50 of DNR combined with 0.25 , 0.5,1.0 mol / L As2O3 on K562/A02 cells were 1.73,0.78,