Double T switch interface with micellar electrokinetic chromatography dimensional gel electrophoresis and capillary zone electrophoresis separation of mixed amino acids on the chip enrichment.docVIP

 PAGE \* MERGEFORMAT 8 Double T switch interface with micellar electrokinetic chromatography dimensional gel electrophoresis and capillary zone electrophoresis separation of mixed amino acids on the chip enrichment Of: Xu Yi Shen Jiwei Cao Mingxia Zhang products [Abstract] design presented in chronological order based on double-T switch interface with micellar electrokinetic chromatography (MEKC) and capillary zone electrophoresis (CZE) two-dimensional chips, constructed the corresponding microarray analysis test system. FITC labeled amino acids based on the sample one-dimensional MEKC and CZE experiments, the injection time on the two-dimensional, two-dimensional separation of start-up time critical operations such as two-dimensional chip electrophoresis parameters were optimized. MEKC CZE constructed using two-dimensional electrophoresis system on chip arginine, lysine, histidine, phenylalanine and tyrosine were mixed samples of two-dimensional chip electrophoretic separation, the calculated two orthogonal separation modes of 56.0%. [Keywords:] two-dimensional chip electrophoresis, micellar electrokinetic chromatography, capillary zone electrophoresis separation of amino acids This article by the National Natural Science Foundation of China (Nos, National Science and Technology 863 Project (No.2006AA04Z345) and the Ministry of International Cooperation Projects (No.2006DFA13510), Chongqing Municipal Natural Science Foundation of China (No.2006BA4012 ) Funding 1 Introduction In the microfluidic chip micro-pipeline network system constructed two-dimensional chip separation is a recently developed separation of complex samples for the analysis of new composite separation technology [1]. Electrophoretic separation of it are two different modes coupled together with the advantages of small size, interface, death, separation system to improve the resolution and peak capacity has demonstrated great potential, making it a complex