苦木化学成分分析.doc

PAGE  PAGE 7 苦木不同部位挥发性化学成分的研究 杨再波1*，郭治友2，龙成梅1，毛海立1，孙成斌1 (1.黔南民族师范学院化学与化工系，贵州 都匀 558000;2. 黔南民族师范学院生命科学系, 贵州 都匀 558000) [摘要] 目的：应用气相色谱-质谱法(GC-MS)分析苦木不同部位的挥发性化学成分。方法：采用微波辅助顶空固相微萃取技术分别提取苦木叶柄、叶和枝的主要挥发性化学成分，用气相色谱-质谱法与Kovats色谱保留指数相结合进行定性定量分析。结果：从苦木的叶柄、叶片和枝中分别鉴定出84、76和60个组分，分别占挥发油总峰面积的97.247 %、97.179%和97.141%，在叶柄中主要成分香芹酚(5.201%)，麝香草酚(6.185%)，反式-丁香烯(6.562%)，α-佛手柑油烯(6.295%)，反-β-金合欢烯(5.088%)，枯茗醇(10.807%)，β-甜没药烯(6.136%)。叶片中主要成分是反式-丁香烯(7.017%)，α-佛手柑油烯(7.848%)，反-β-金合欢烯(8.297%)，枯茗醇(10.728%)，β-甜没药烯(7.150%)，(-)-石竹烯氧化物(5.094%)。而在枝中主要是反式-丁香烯(5.530%，α-佛手柑油烯(9.997%)，反-β-金合欢烯(7.994%)，枯茗醇(15.416%)，β-甜没药烯(12.733%)。结论:苦木叶柄、叶和枝所含挥发性成分差异较大。 关键词：苦木；挥发性成分；微波辅助顶空固相微萃取；气相色谱-质谱 Study of Volatile chemical constituents in Different Parts of Picrasma quassioides (D.Don) Benn. Yang Zaibo1*，Guo Zhiyou2, Long Chengmei1 , Mao Haili1,Sun Chengbin1 （1.Department of Chemistry and Chemical Engineering, Qiannan Normal College for Nationalities, Duyun GuiZhou 558000, China;2. Department of Life Sciences, Qiannan Normal University for Nationalities, Duyun ,Guizhou, 558000） ［Abstract］ Objective: To analyze and compare the volatile constituents which were extracted from the different parts of Picrasma quassioides (D.Don) Benn. Method: The volatile components were extracted from the different parts of Picrasma quassioides (D.Don) Benn. by the methods of MAE-HS-SPME and the chemical components of them were identified by GC-MS and Kovats retention index. Result: 84 volatile constituents in the petiole, 76 in the leaf blade and 60 in the branch of Picrasma quassioides (D.Don) Benn. were separated and identified. accounting for 97.247 %、97.179% and 97.141% of the total peak areas, respectively. Which is the main component in petiole carvacrol (5.201%)，thymol (6.185%)，trans-caryophyllene (6.562%)，alpha-bergamotene (6.295%)，trans-beta-farnesene (5.088%)， cuminol (10.807%)， beta-bisabolene 6.136%). And in the leaf blade trans-caryophyllene (7.017%)，alpha-bergamotene (7.848%)，trans-beta-farnesene (8.297%)， cumi