柑橘中多菌灵的提取及薄层色谱研究.docVIP

邵阳学院2014届毕业论文  PAGE \* MERGEFORMAT I 毕业论文 课 题 名 称 柑橘中多菌灵的提取及薄层色谱研究 ——乙腈作提取剂 学 生 姓 名 胡 恒 学 号 1040904091 系、年级专业 生物与化学工程系10食品质量与安全 指 导 教 师 夏 湘 职 称 副 教 授 2014年5月26日 柑橘中多菌灵的提取及薄层色谱研究 胡 恒 （邵阳学院生物与化学工程系食品质量与安全专业 邵阳 422000） 指导老师：夏 湘 摘 要 目的：以乙腈做提取剂，采用水浴超声、机械震荡等方法提取，利用薄层层析分离净化，研究探讨并建立比较简单的柑橘中多菌灵的提取、净化操作条件，为农残的检测提供理论依据，并初步学会科学研究方法，提高实践能力。方法：以乙腈做提取剂，采用水浴超声、机械震荡等方法提取，通过薄层色谱确定柱层析合适的洗脱剂。结果：通过实验得到柑橘中多菌灵的较为合适的提取、净化条件。结论：本方法廉价、快速、操作简便、提取净化效果较好，可以满足食品工业分析要求。 关键词：多菌灵；提取；净化；薄层色谱  PAGE \* MERGEFORMAT II Study on Extraction and thin-layer chromatography of spirit in Citrus Hu Heng (Department of Biology and Chemistry Engineering, Shaoyang University, Shaoyang 422000, China) Counselor:Xia Xiang Abstract Objective:Using acetonitrile as extractant, extraction by water bath andultrasonic, mechanical shock method, separation and purification by TLC, to study and to establish a relatively simple orange carbendazim in the extraction,purification and operation conditions, to provide the theoretical basis for thedetection of pesticide residues, and initially learn scientific research methods,improve the practice ability. Methods: using acetonitrile as extractant, extractionby water bath and ultrasonic, mechanical shock and other methods, by thin layer chromatography to determine the appropriate eluent column chromatography.Results: the orange carbendazim extraction, suitable purification conditionexperiment. Conclusion: The method is cheap, fast, convenient operation,extraction and purification effect is good, Which can meet the requirements of the food industry analysis. Keywords: Carbendazim; extraction; purification; thin layer chromatography  PAGE