EDFS40 of cryopreserved rabbit carotid artery structure and systolic and diastolic function of.docVIP

 PAGE \* MERGEFORMAT 13 EDFS40 of cryopreserved rabbit carotid artery structure and systolic and diastolic function of Of: Jiang An, Liu Feng, Liu, Song Yulong, Meng Kb Wei, Lu Yi Jiang An [Abstract] Objective To evaluate the vitrification solution EDFS40 of cryopreserved rabbit carotid artery morphology and systolic and diastolic function. Methods of rabbits under sterile conditions, carotid artery, respectively EDFS40 and 1.5mol / L dimethyl sulfoxide as cryoprotectant, after vitrification and slow freezing cryopreservation 14d. artery after rewarming and fresh for gross and microscopic morphology observation, within the organ bath of non-determination of endothelium-dependent relaxation and contraction. Results EDFS40 group artery gross and histologic and ultrastructural similarities with fresh arteries, the contractile force of potassium chloride and slow freezing was no significant difference between groups arteries (Pgt; 0.05), norepinephrine and sodium nitroprusside on the contraction or relaxation tension than the latter (P lt;0.05), but lower than the fresh arteries (P lt;0.05). Conclusion EDFS40 can better maintain the shape of rabbit carotid artery structure and function of the glass can be used to save the vessel. [Keywords:] cryopreservation, rabbit, carotid artery, EDFS40, glass ABSTRACT: Objective To assess the effect of EDFS40 on the structure and vasomotoricity of rabbit carotids in cryopreservation. Methods The fresh carotids, taken from rabbits under aseptic conditions, were preserved for 14 days in liquid nitrogen by vitrification or slow freezing method with EDFS40 or dimethyl sulfoxide (1.5mol / L). The morphological changes of both fresh and thawed carotids were observed by visual examination, optical and electron microscopes, respectively. Their endothelium independent vasoconstriction and vasodilatation were detected in organ bath. Results The morphological changes of the EDFS40 vitrified arteries were similar to those of th