ELISPOT technology in the diagnosis of tuberculous meningitis in children.docVIP

 PAGE \* MERGEFORMAT 8 ELISPOT technology in the diagnosis of tuberculous meningitis in children Discuss ELISPOT technique (ELISPOT) in the diagnosis of tuberculous meningitis in children in the application. Methods: 0 ~ 14-year-old children with suspected tuberculous meningitis were 17 cases for ELISPOT testing, the final analysis confirmed cases, and with head CT / MRI examination, tuberculosis purified protein derivative test (PPD) test, cerebrospinal fluid examination , TB antibody test, chest X-ray results compared. Results: In the final confirmed 13 cases of tuberculous meningitis, cranial CT / MRI, PPD, cerebrospinal fluid, tuberculosis antibody, chest X-ray at the hospital within 5 d-positive rates were 30.8%, 30.8%, 23.1%, 12.5% , 30.8%, while the ELISPOT positive rate was 76.9%, significantly higher than other auxiliary examination methods (P amp;lt;0.05). Conclusion: ELISPOT check the sensitive, rapid, and for children with tuberculous meningitis there is an early diagnosis. Dot-ELISA technique (enzyme-linked immunospot assay, ELISPOT) is a secretion from a single cell level detection of antibodies or immune cells, cytokines learn new techniques [1,2]. In recent years, based on two kinds of M. tuberculosis-specific antigens early secretory antigen target-6 (early secretory antigen target-6, ESAT-6), culture filtrate protein 10 (culture filtrate protein-10, CFP-10) in ELISPOT technology, with its sensitive, specific, simple and the advantages of being used for TB diagnosis, and achieved good results [2 ~ 5], in Europe and the United States has been approved for clinical use, but is still in the experimental stage in China. Section I of the technology will be tested in children with tuberculous meningitis (tuberculous meningitis) in the diagnosis, and other routine laboratory examinations done comparison of ELISPOT in the diagnosis of pediatric brain knot. 1 Data and methods 1.1 The clinical data collection, our department in September 2006 ~ Septe